Low-density Lipoprotein Improves Motility and Plasma Membrane Integrity of Cryopreserved Canine Epididymal Spermatozoa
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چکیده
منابع مشابه
Low-density Lipoprotein Improves Motility and Plasma Membrane Integrity of Cryopreserved Canine Epididymal Spermatozoa.
Cryopreservation of caudal epididymal spermatozoa is an effective technique to conserve genetic potentials of superior dogs when it is not possible to collect ejaculated spermatozoa. Although hen egg yolk is commonly supplemented into the semen extender, active substances within the egg yolk which protect sperm against cryoinjury remain to be discovered. Among its compositions, low-density lipo...
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The present study was designed to test the hypothesis that addition of anticaspase cocktails (inhibiting caspases and thus blocking apoptosis) to the extenders increases the post-thaw viability of equine spermatozoa. The addition of caspase inhibitors failed to improve the acrosome and plasma membrane integrity of spermatozoa, suggesting that in equine sperm cryopreservation protocols, the addi...
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Semen samples from 9 sexually mature dogs of different breeds, aged from 1 to 5 years were used. Quality of sperm was evaluated using the following parameters: progressive motility (subjective), then structural and functional integrity of spermal membrane (supravital colouring (eosine/nigrosine) and HOS test). The majority of HOS reactive or swollen spermatozoa was placed in a hypoosmolar solut...
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Background: Hen egg yolk is a common component of most semen cryopreservation extenders. However, diluents containing the egg yolk may have adverse effects on the quality of the semen. Egg yolk could be solubilized by the addition of sodium dodecyl sulphate (SDS) in semen extenders. Many studies have shown beneficial effects of antioxidants addition to semen freezing extenders. Antioxidants are...
متن کاملMotility and Membrane Integrity of Ejaculated Bovine Spermatozoa Extended and Cryopreserved in L-carnitine Tris-egg Yolk Extender
This study was conducted to examine if L-carnitine supplementation in the Tris-egg-yolk extender can improve the motility characteristics, membrane integrity, and in vitro fertilization potential after cryopreservation of ejaculated bovine spermatozoa. It was carried out on January to May 2013 at the University of Wisconsin-Madison. L-carnitine at final concentrations of 0.5, 1, 10, and 30mM we...
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ژورنال
عنوان ژورنال: Asian-Australasian Journal of Animal Sciences
سال: 2015
ISSN: 1011-2367,1976-5517
DOI: 10.5713/ajas.15.0572